5-Fluoroorotic Acid (5-FOA) High-Throughput/Automated Isolation RNA transcription

RNA transcription

is suitable for hybridization

DNA/RNA is washed and concentrated into a small volume of water

arrest the cell cycle in G1

5-Fluoroorotic Acid (5-FOA) High-Throughput/Automated Isolation RNA transcriptionUsing 5 Fluoroorotic Acid (5 FOA) for the counter selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two hybrid screens are methods that can employ the use of 5 FOA. Otherwise nontoxic to yeast, 5 FOA is converted to the toxic form (i. e., 5 flurouracil) in strains expressing the functional URA3 gene coding for orotine 5 monophosphate decarboxylase that is involved in